WebThe QIAGEN Multiplex PCR Kit is highly suited for various multiplex applications, including: Typing and analysis of transgenic organisms Amplification and analysis of microsatellites Typing and detection of bacteria and viruses Amplification of multiple DNA regions for SNP analysis Supporting data and figures Successful 16-plex PCR. WebThe TaqMan real-time RT-PCR assays were performed using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific). Each 20 μl reaction mix contained 5 μl of 4× Fast Virus 1-Step Master Mix, 0.2 μl of 50 μM probe, 0.2 μl each of 50 μM forward and reverse primers, 12.4 μl of nuclease-free water, and 2 μl of extracted RNA.
TaqMan™ Reagents Starter Kit
WebThese kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g., TaqMan ®), FRET probes, and displacement probes (e.g., molecular beacons). Compatibility with probe-based qPCR applications and instruments WebiTaq Universal One-Step RT-qPCR Kits are a fast and convenient solution for real-time PCR using the powerful combination of iScript RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA Polymerase in one reaction, providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance without … prefix with sexual nyt crossword
qPCR/Real-Time PCR Reagents Bio-Rad
WebA TaqMan probe-based qPCR reaction contains the following components: PCR master mix DNA template Primers Probe (s) Preformulated PCR master mixes containing buffer, … WebTECHNOLOGY; MANUAL; SDS; PRINT. DESCRIPTION. Hot Start TTx (DNA) Kit is qPCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA Polymerase … WebThis kit includes the following components for 250 reactions, 20 μL total reaction volume. All reagents should be stored at -20°C. < HSTTX-101 >. 2x Buffer for rTth/ TTx (DNA) 1.25 mL x 2. Hot Start TTx DNA Polymerase (4U/ μL) 62.5 μL. Note: 2x Reaction Buffer contains essential components for the reaction (buffer, salts, Mg2+, dATP, dCTP ... scotcheroos my mother in law\\u0027s recipe